The aerobiome uncovered: Multi-marker metabarcoding reveals potential drivers of turn-over in the full microbial community in the air (2024)

de Groot, G. A., Geisen, S., Wubs, E. R. J., Meulenbroek, L., Laros, I., Snoek, L. B., Lammertsma, D. R., Hansen, L. H., & Slim, P. A. (2021). The aerobiome uncovered: Multi-marker metabarcoding reveals potential drivers of turn-over in the full microbial community in the air. Environment international, 154, 1-8. Article 106551. https://doi.org/10.1016/j.envint.2021.106551

de Groot, G Arjen ; Geisen, Stefan ; Wubs, E R Jasper et al. / The aerobiome uncovered : Multi-marker metabarcoding reveals potential drivers of turn-over in the full microbial community in the air. In: Environment international. 2021 ; Vol. 154. pp. 1-8.

@article{2e508e96f26747b8b335ca9dca0891ec,

title = "The aerobiome uncovered: Multi-marker metabarcoding reveals potential drivers of turn-over in the full microbial community in the air",

abstract = "Air is a major conduit for the dispersal of organisms at the local and the global scale. Most research has focused on the dispersal of plants, vertebrates and human disease agents. However, the air represents a key dispersal medium also for bacteria, fungi and protists. Many of those represent potential pathogens of animals and plants and have until now gone largely unrecorded. Here we studied the turnover in composition of the entire aerobiome, the collective diversity of airborne microorganisms. For that we performed daily analyses of all prokaryotes and eukaryotes (including plants) using multi-marker high-throughput sequencing for a total of three weeks. We linked the resulting communities to local weather conditions, to assess determinants of aerobiome composition and distribution. We observed hundreds of microbial taxa, mostly belonging to spore-forming organisms including fungi, but also protists. Additionally, we detected many potential human- and plant-pathogens. Community composition fluctuated on a daily basis and was linked to concurrent weather conditions, particularly air pressure and temperature. Using network analyses, we identified taxonomically diverse groups of organisms with correlated temporal dynamics. In part, this was due to co-variation with environmental conditions, while we could also detect specific host-parasite interactions. This study provides the first full inventory of the aerobiome and identifies putative drivers of its dynamics in terms of taxon composition. This knowledge can help develop early warning systems against pathogens and improve our understanding of microbial dispersal.",

keywords = "Aerobiome monitoring, Co-dispersal, Early-warning system, Meteorology, Microbial dispersal, Microbiome, Potential pathogens",

author = "{de Groot}, {G Arjen} and Stefan Geisen and Wubs, {E R Jasper} and Liz Meulenbroek and Ivo Laros and Snoek, {L Basten} and Lammertsma, {Dennis R} and Hansen, {Lars H} and Slim, {Pieter A}",

note = "Copyright {\textcopyright} 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved. Funding Information: This research was part of the KennisBasis IV strategic research program {\textquoteleft}Sustainable Spatial Development of Ecosystems, Landscapes, Seas and Regions{\textquoteright} (KB-24-002-005), funded by the Dutch Ministry of Economic Affairs and carried out by Wageningen University & Research. SG was supported by an NWO-VENI grant from the Netherlands Organization for Scientific Research (016.Veni.181.078). ERJW was funded by an NWO-RUBICON grant (Netherlands Organization for Scientific Research, contract no. 019.181EN.01) and by ERC-ADV 323020 SPECIALS to W.H. van der Putten. The funding bodies had no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript. Publisher Copyright: {\textcopyright} 2021 The Author(s)",

year = "2021",

month = sep,

doi = "10.1016/j.envint.2021.106551",

language = "English",

volume = "154",

pages = "1--8",

journal = "Environment international",

issn = "0160-4120",

publisher = "Elsevier Limited",

}

de Groot, GA, Geisen, S, Wubs, ERJ, Meulenbroek, L, Laros, I, Snoek, LB, Lammertsma, DR, Hansen, LH & Slim, PA 2021, 'The aerobiome uncovered: Multi-marker metabarcoding reveals potential drivers of turn-over in the full microbial community in the air', Environment international, vol. 154, 106551, pp. 1-8. https://doi.org/10.1016/j.envint.2021.106551

The aerobiome uncovered: Multi-marker metabarcoding reveals potential drivers of turn-over in the full microbial community in the air. / de Groot, G Arjen; Geisen, Stefan; Wubs, E R Jasper et al.
In: Environment international, Vol. 154, 106551, 09.2021, p. 1-8.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - The aerobiome uncovered

T2 - Multi-marker metabarcoding reveals potential drivers of turn-over in the full microbial community in the air

AU - de Groot, G Arjen

AU - Geisen, Stefan

AU - Wubs, E R Jasper

AU - Meulenbroek, Liz

AU - Laros, Ivo

AU - Snoek, L Basten

AU - Lammertsma, Dennis R

AU - Hansen, Lars H

AU - Slim, Pieter A

N1 - Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved.Funding Information:This research was part of the KennisBasis IV strategic research program ‘Sustainable Spatial Development of Ecosystems, Landscapes, Seas and Regions’ (KB-24-002-005), funded by the Dutch Ministry of Economic Affairs and carried out by Wageningen University & Research. SG was supported by an NWO-VENI grant from the Netherlands Organization for Scientific Research (016.Veni.181.078). ERJW was funded by an NWO-RUBICON grant (Netherlands Organization for Scientific Research, contract no. 019.181EN.01) and by ERC-ADV 323020 SPECIALS to W.H. van der Putten. The funding bodies had no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.Publisher Copyright:© 2021 The Author(s)

PY - 2021/9

Y1 - 2021/9

N2 - Air is a major conduit for the dispersal of organisms at the local and the global scale. Most research has focused on the dispersal of plants, vertebrates and human disease agents. However, the air represents a key dispersal medium also for bacteria, fungi and protists. Many of those represent potential pathogens of animals and plants and have until now gone largely unrecorded. Here we studied the turnover in composition of the entire aerobiome, the collective diversity of airborne microorganisms. For that we performed daily analyses of all prokaryotes and eukaryotes (including plants) using multi-marker high-throughput sequencing for a total of three weeks. We linked the resulting communities to local weather conditions, to assess determinants of aerobiome composition and distribution. We observed hundreds of microbial taxa, mostly belonging to spore-forming organisms including fungi, but also protists. Additionally, we detected many potential human- and plant-pathogens. Community composition fluctuated on a daily basis and was linked to concurrent weather conditions, particularly air pressure and temperature. Using network analyses, we identified taxonomically diverse groups of organisms with correlated temporal dynamics. In part, this was due to co-variation with environmental conditions, while we could also detect specific host-parasite interactions. This study provides the first full inventory of the aerobiome and identifies putative drivers of its dynamics in terms of taxon composition. This knowledge can help develop early warning systems against pathogens and improve our understanding of microbial dispersal.

AB - Air is a major conduit for the dispersal of organisms at the local and the global scale. Most research has focused on the dispersal of plants, vertebrates and human disease agents. However, the air represents a key dispersal medium also for bacteria, fungi and protists. Many of those represent potential pathogens of animals and plants and have until now gone largely unrecorded. Here we studied the turnover in composition of the entire aerobiome, the collective diversity of airborne microorganisms. For that we performed daily analyses of all prokaryotes and eukaryotes (including plants) using multi-marker high-throughput sequencing for a total of three weeks. We linked the resulting communities to local weather conditions, to assess determinants of aerobiome composition and distribution. We observed hundreds of microbial taxa, mostly belonging to spore-forming organisms including fungi, but also protists. Additionally, we detected many potential human- and plant-pathogens. Community composition fluctuated on a daily basis and was linked to concurrent weather conditions, particularly air pressure and temperature. Using network analyses, we identified taxonomically diverse groups of organisms with correlated temporal dynamics. In part, this was due to co-variation with environmental conditions, while we could also detect specific host-parasite interactions. This study provides the first full inventory of the aerobiome and identifies putative drivers of its dynamics in terms of taxon composition. This knowledge can help develop early warning systems against pathogens and improve our understanding of microbial dispersal.

KW - Aerobiome monitoring

KW - Co-dispersal

KW - Early-warning system

KW - Meteorology

KW - Microbial dispersal

KW - Microbiome

KW - Potential pathogens

UR - http://www.scopus.com/inward/record.url?scp=85103936557&partnerID=8YFLogxK

U2 - 10.1016/j.envint.2021.106551

DO - 10.1016/j.envint.2021.106551

M3 - Article

C2 - 33857708

SN - 0160-4120

VL - 154

SP - 1

EP - 8

JO - Environment international

JF - Environment international

M1 - 106551

ER -

de Groot GA, Geisen S, Wubs ERJ, Meulenbroek L, Laros I, Snoek LB et al. The aerobiome uncovered: Multi-marker metabarcoding reveals potential drivers of turn-over in the full microbial community in the air. Environment international. 2021 Sept;154:1-8. 106551. doi: 10.1016/j.envint.2021.106551